Nope, still not it.

If 46 is right, I'll feel cheated since the game is named Super Mario Galaxy! 

Soon I'll have to reveal my first game, so go on, try to guess it. By the way, my list contains quite a few new entries, but mostly in the lower part of the list. As a result #50, #49 and #48 all are new entries in my list.

#50
Play the cards and roll the dice!
The dev is now owned by Microsoft.
A pen&paper RPG converted to a card game as a video game.
Manage your own party to find your path in this adventure.

#49
Sally looks for Emily.
A nightmare.
Play at the Bachman school, an arcade and a mine.

#48
Beat that dragon with the help of your circular companion.
Get your workout to beat the enemies.

#47
Both princesses are actually featured in the title of the game.

47 - TLOZ: Twilight Princess?

Wow, that was fast. And correct too!

Ring Fit Adventure

Surprised this game makes it in a top 50 all time! I'm curious to see if it's gonna be in the overall top when all is said and done!

Correct on #50, and a good guess for 46 but not quite. You have the right word there though.

Correct. And well, I think it is important enough to earn a spot at least for one year. Nintendo did quite well with this and I am not against non-traditional games. For the overall toplist... mZuzek compiles a list including all games listed anywhere. So it will be included, but probably with a rank somewhere 500-1000 or so.

I take it its Rogue Galaxy then, right? 

OK, two games are guessed, but not the first two I have to reveal. So I give another hint to #50, so that hopefully someone gets it.

#50
Play the cards and roll the dice!
The dev is now owned by Microsoft.
A pen&paper RPG converted to a card game as a video game.
Manage your own party to find your path in this adventure.
This mobile game features the first adventure path of a series, happening in Varisia starting with a goblin adventure in Sandpoint.

#49
Sally looks for Emily.
A nightmare.
Play at the Bachman school, an arcade and a mine.
There is no working Tequila as far as I know in the game, but Soda cans that can be used to distract monsters.

#48: Ring Fit Adventure as guessed by Supermario28.

#47: The Legend of Zelda: Twilight Princess as guessed by Jpcc86.

#46
Craft to survive and try to escape.