Star Wars (film) -- When Darth Vader killed that officer with a force choke, I was hooked. That really set up the antagonists for the film and I think it would have been a less effective story without it. Plus it was just so freaking cool.
Hyperion (novel) -- The introduction of the Shrike. When it appears, it just wrecks everything. It is literally unbeatable and a threat that defines much of the first book.
from Wikipedia:
"Surrounded in mystery, the object of fear, hatred, and even worship by members of the Church of the Final Atonement (the Shrike Cult), the Shrike's origins are described as uncertain. It is portrayed as composed of razorwire, thorns, blades, and cutting edges, having fingers like scalpels and long, curved toe blades. It has the ability to control the flow of time, and may thus appear to travel infinitely fast. The Shrike may kill victims in a flash or it may transport them to an eternity of impalement upon an enormous artificial 'Tree of Thorns,' or 'Tree of Pain' in Hyperion's distant future. The Tree of Thorns is described as unimaginably large, metallic tree, alive with the agonized writhing of countless human victims of all ages and races."
Dune (novel) -- When they take the coptor-thing out to the Spice factory, which gets attacked by a Sand Worm. That marked the Sand Worms as forces of nature that shaped and controled the entire planet, despite all of mankind's relative power.
Alien (film) -- The scene with the flamethrower in the shaft. One of the most tension-filled moments in cinema history. Absolutely brilliant.
This Day All Gods Die (novel) -- There is a moment when the events of three novels turn full-circle that is pretty epic. Angus had raped, abused, and nearly broken Morn. Through the use of an illegal cybernetic implant, he had turned her into a literal slave. He had come to both hate and love her. After being broken himself, and having learned some humanity, in the third book, also through the use of illegal cybernetic implants, he ends up in a position where he cannot physically disobey Morn. Suddenly he is her slave and the narrative turns from her being carried along by events to actually focusing on what she wants to accomplish. It's a lot more complicated than that, of course, with both being trapped in an incredibly desperate situation, but that switch in perspective is major part of what the novels are all about. There is so much in play here, including how much both characters have changed after what they've gone through.
Neuromancer (novel) -- When we're shown a real over-view of the world inside the novel. For me, this was what defined cyberpunk. It was a brilliant combination of reality and science-fiction. It was seedy and dark, where once sci-fi had been clean and bright. It was like our own world, with all the uglyness, only with more technology. This changed science fiction for me as a genre.