Battletoads or Kirby from that list.

battletoads pushed the 16bit envelope almost.

kirby was just a really pretty NES game at end of Gen.

Is there one that's not on the list you'd like to give a shoutout?

Decent choices, but shout outs to the Flintstones and the Jungle Book are in order.  Seriously, when I was a kid those games blew my mind and eased the pain into the 16 bit era.

The Immortal for good iso-perspective - honestly, it could've fooled as being an early Amiga game. But overall, probably Battletoads, as Cobretti said, game really pushed into 16-bit territory like no other NES game.

A couple of games that need mentioning:

Castlevania 3

Little Nemo

River City Ransom

Kirby's Adventure was beautiful.

Rescue had some nice graphics loved to sniper bad guys.

Man, you're on a roll with these 'best - ' threads. I think you've covered other gens as well? I can't recall them all.

Anyways, I didn't play a lot of NES games, so I'll go with Mario Bros 3 O:

http://gamrconnect.vgchartz.com/thread.php?id=200595&page=1