Tomb Raider (1996) - Palace Midas
A massive, expansive level that is great fun to play through
Tomb Raider II - Opera House, The Deck
Opera House was like delving into the heart of enemy territory, and is a fantastic setting, whereas The Deck is much like Palace Midas above. I look forward to these levels on my annual TR2 run.
Paper Mario: The Thousand Year Door - Chapter 3 (Glitzville)
Someone already mentioned this. Incredible chapter that had me gripped the first time I played it. I was up until the early hours completing it as I had to know what was going on!
Super Mario 64 - Lethal Lava Land
Purely for the shell surfing.
The Legend of Zelda: Twilight Princess - Snowpeak Ruins
Someone else mentioned a Zelda dungeon, so I will too. I liked the atmosphere to this one, gradually exploring the Yeti's home.
Sonic the Hedgehog (Master System) - Jungle Act 1
Always the highlight when I play. I particularly enjoy the jovial music.
Super Mario Galaxy - Beach Bowl Galaxy
Bright, vibrant and fun.
Banjo Kazooie - Treasure Trove Cove
See Mario Galaxy above.
Super Mario Odyssey - Luncheon Kingdom
Early days with this game yet, but this level had me absolutely hooked.
Goldeneye - Surface
Running round killing enemies in the snow with all weapons and infinite ammo never gets old.