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Ask me another day and I could probably list a completely different 5, in no particular order.

Matrix: "He is beginning to believe."

Metroid Prime: the part between descending onto Tallon IV's surface and when you beat Metroid Prime.

Serenity (River): "You've always take care of me. My turn."

Battlestar Galatica: the whole thing, for capturing and sustaining the feeling the entire human race is hanging over a precipice, and could fall at any moment.

Hitchhiker's Guide to the Galaxy (books): too hard to think of a favourite bit, there are too many.