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Dr.Henry_Killinger said:

Origin of Mario and Luigi: Mommy and Daddy did it (But, if that's not what you meant, then they were Plumbers and a pipe led them to the Mushroom Kingdom. Haven't you watched the TV show?)

Origin of The Princesses: Ditto.

Conflict between Bowser/Koopas and the Mushroom Kingdom: Bowser wants to be King and he wants Peach to be his Wife. He's angry that no one lets him have a chance with her, so he kidnaps her.

The responsibility of the Princesses: Peach is the ruler of her kingdom... What else?

Mushrooms: Drugs.

Yoshis: A different life-form that happens to live in the Mushroom Kingdom.