1. Mario
2. Luigi
3. Donkey Kong.
4. Kirby Pac Man
5. Sonic
6. Miles "Tails" Prower
7. Crash Bandicoot
8. Sackboy
9. Banjo w/ Kazooie
10. Conker the Squirrel
11. S'plosion Man Ray Man
12. Mega Man
13. Ratchet w/ Clank
14. Jak w/ Daxter
and to break up this sausage fest,
15. Kameo
16. Amy Rose
17. Roll (from Mega Man)
18. Princess Peach
19. Shantae
20. Patricia Wagon (from Mighty Switch Force)
*edit* looking at other people's lists, I need more icons. I'd dump Kirby for Pac-Man and 'Splosion Man for Rayman. I'm trying to go with all cartoony art style. I always disliked when a realistic Samus would clash with a cartoon Luigi or something. I'm trying to keep the Nintendo guys to a minimum, too. That's why Toon Link didn't make the cut.
