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No clear objective at all times. No outside support, no rails missions. No real story at all except what the player makes through their actions and journey.
Less linearity than Prime 3; all on one planet; interconnected and surprising world ability to speed run and sequence break.
Fewer meaningless fetchquests than Prime 1-3
Kenji Yamamoto sole composer (unlike Prime 3 and Other M)
Absolutely nothing from Other M - no cutscenes, no anime story, no anime music, no feminine side. Samus is a shell for the player.
Absolutely nothing from Halo or related sci-fi shooters. Metroid is not a shooter. No co-op, no achievements, no multiplayer, no DLC, no patches, no social features, again no cutscenes or story, no escort missions.
Option for no motion control - did not like change of controls in Prime 3.