Boromir's Death (The Lord of the Rings: The Fellowship of the Ring)
Aragorn: I will not let the White City Fall. Nor our people fail...
Boromir: Our people... our people...
*grabs dagger*
I would have followed you my brother. My Captain. My king.
Aragorn: Be at peace, son of Gondor.
Gets me every single time. *deep sigh*
EDIT: Just watched the Up scene right after this. It's like I've been punched in the face by the fist of sadness.