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I have played the 3DS for 2-5 hours straight with no  headaches or anything like that. With anything you have to ease yourself into it. First when I got, I had the slider up about 1/2. The more and more I played it, the higher the slider would go. Most games now, it's on full blast or just a smidge before.

Except for Rayman 3D, I just turned the whole console off :)

Everyone is different. According to everyone. That is why there is the slider to adjust. So, adjust accordingly.