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I've only played on our interactive at work and don't currently own one but I've played for up to 30 minutes at a time several times.  I have noticed slight headache and eye strain from it but no nausea or anything. 

The 3D effect is pretty much amazing and has blown me away but I'm somewhat cautious of buying one due to the slight symptoms I've had in short periods of play time.  Anyone know if this is something you get used to after a while and it goes away or will the 3D always give me a headache?