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I dunno if it's a discovery or not... but after playing through the game again I'm starting to think the Nicole on the ship during the end was indeed real and NOT and illusion.

Nicole gets on the shuttle before stage 12 with Isaac and after she tells Isaac to move the marker off the ship, Isaac goes into the room to collect that battery and she probably went back into the shuttle at that point.

Also, if Nicole was an illusion the ENTIRE time, she would not have been able to open doors or use the computer at key moments during the game. I believe the Hivemind was using her body throughout the game and when it died, she began to think on her own and for some reason attacks Isaac. (or attempts to kiss him at the end) ^_^.