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Forums - General - How to calculate LC50 toxicity value?

dany612 on 26 January 2011

Hi,

I have a question and I hope Vgchartz can help me. I am trying to determine and calulate the LC50 value for a sendiment sample. I am using water fleas (Daphnia Magna) as a experimental variable. 

 

Hopefully I will find a source explaining this better but in the meanwhile I thought perhaps I'll check here first.



 

        

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dany612 on 26 January 2011
trunkswd said:

I haven't heard the term before, but doing a quick search it is very similar to LD50. I'm assuming you know what LC50 is testing for. I'm exactly sure what your experiment is, but I know for testing LD50 you put the specimens into different chambers and give each one of them different dosages of a toxin and see how long it takes for 50% of the specimens to die for each chamber. I've never done it but did talk about it in my watershed science class last semeter.


Yes it is very similiar. I have one question since I hope you do know. What is Log10 concentration? I am looking at this data and they don't expand on it. 



 

        

dany612 on 26 January 2011
trunkswd said:
dany612 said:
trunkswd said:

I haven't heard the term before, but doing a quick search it is very similar to LD50. I'm assuming you know what LC50 is testing for. I'm exactly sure what your experiment is, but I know for testing LD50 you put the specimens into different chambers and give each one of them different dosages of a toxin and see how long it takes for 50% of the specimens to die for each chamber. I've never done it but did talk about it in my watershed science class last semeter.


Yes it is very similiar. I have one question since I hope you do know. What is Log10 concentration? I am looking at this data and they don't expand on it. 

I know what a log (logarithm) is but I haven't come across Log10 concentration before. I suggest to look in your class textbook or to ask a classmate. I hope you figure it out.

I did. Now I know. One last thing and hopefully I find out soon. I have this sendiment sample that is .8 inches in lengh. Do you know how that can be converted to mg/l?



 

        

dany612 on 26 January 2011
trunkswd said:
dany612 said:
trunkswd said:
dany612 said:
trunkswd said:

I haven't heard the term before, but doing a quick search it is very similar to LD50. I'm assuming you know what LC50 is testing for. I'm exactly sure what your experiment is, but I know for testing LD50 you put the specimens into different chambers and give each one of them different dosages of a toxin and see how long it takes for 50% of the specimens to die for each chamber. I've never done it but did talk about it in my watershed science class last semeter.


Yes it is very similiar. I have one question since I hope you do know. What is Log10 concentration? I am looking at this data and they don't expand on it. 

I know what a log (logarithm) is but I haven't come across Log10 concentration before. I suggest to look in your class textbook or to ask a classmate. I hope you figure it out.

I did. Now I know. One last thing and hopefully I find out soon. I have this sendiment sample that is .8 inches in lengh. Do you know how that can be converted to mg/l?


.8 inches by X what? You need the volume of it to convert it to mg/L.

That's how I am getting confused. It's about 6 grams, sediment sample. The whole container i put it in is 7 fluid onces. 

Edit: how would i find the volume. 



 

        

Pyro as Bill on 28 January 2011

 

The 7 fluid ounces is the volume (200ml).

6g = 6000mg

30,000mg/L or 30,000ppm



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