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With bathroom breaks and 5-10 minutes to eat, 15 hours. It was an endless shareware strategy game where you couldn't save, so you just had to keep going if you wanted to make most of what you had build.
My buddy and I were kind of competing on finishing management strategy game Detroit, so at one point I did 14 hours on that.
And my third longest is Dune 2 with something like 12 hours.
I'm sure one or other Civilization gets to same category, but that's with more breaks and doing stuff on the side.

On actual one sitting with no break whatsoever, I think I have at some point played Pokemon for 7-8 hours.

On action games with no breaks over 10 seconds, I have played Master System Hang-On and PC version of old Star Wars Arcade game for over 3 hours straight on.