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I would say the dogs in the first Resident Evil. Not only are you screaming and jumping out of your screen, you're also franticly trying to mash buttons in an attempt to save your life lol.

On a side note though--I've found, strangely enough, that the better the graphics, the less scary a game is. I was far more creeped out by Silent Hill and Resident Evil when they were on PS1 than I was when they on more advanced systems. The Gamecube port being a prime example of this. Yes, it looks a billion times better. But is it as scary? As atmospheric? I personally didn't think so. There is something about having to fill in the blanks, so to speak, that makes games of this nature more effective.

But maybe that's just me?