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In 2D games, probably the Screw Attack. Once you have the space jump you can basically fly, but once you have the Screw Attack you're an unstoppable monster. In 3D games, the Spider Ball is pretty awesome, but I feel the Plasma Beam in Metroid Prime deserves mention. Remember the first time you vaporized a Sheegoth?

https://youtu.be/ylHgeartFs8?t=686

I mean I don't know about you, but while I was expecting it to be effective, I wasn't expecting it to be that satisfying.