It was technically second party (Silicon Knights), but that bathtub scene in Eternal Darkness.. Nintendo published it, so close enough I guess.
That weird part in Earthbound where you go inside your evil thoughts or whatever.
Maybe that creepy sun chasing you in Mario 3, especially in world 8. It's actually a pretty frightening concept if you think about it.
Tingle. Just Tingle in general.
"We hold these truths to be self-evident - all men and women created by the, go-you know.. you know the thing!" - Joe Biden