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Epic Mickey

EarthBound

Super Paper Mario (That river with the hands and really creepy music)

Ocarina of Time (The Well, Shadow Temple)

Psychonauts (Secret room in Milla's mind, the asylum)

Pokémon Platinum (Old Chateau)

Pokémon Gold (The Union Cave music creeped me out as a kid, it's not actually scary though)

Donkey Kong 64 (Frantic Factory)

Super Mario 64 (Haunted House, especially that freaking piano!)

There are probably more, but I don't remember right now. If I remember later I'll edit my post.

EDIT: I'm starting to see a pattern here, why does Nintendo put so many creepy things in their games? lol