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1. Mars Matrix (DC) - When I finally beat the game on the default settings. I had practiced almost every day for about 10 months before I finally pulled it off. I was on my last life too. My entire body was all tingly when it happened.
2. Super Metroid (SNES) - There was that one special moment during the Mother Brain battle that took me by surprise and totally floored me. This is the only goosebump moment I can think of that isn't related to music or overcoming a difficult challenge.
3. Rock Band (360) - Beating Green Grass and High Tides on Expert guitar. This song tortured me, but I eventually got my revenge.
4. The Legend of Zelda: Ocarina of Time (N64) - The first time I heard the music during the ending sequence I got goosebumps.
5. Mega Man 9 (Wii) - The first time I selected a stage and heard that classic "Game Start" music I got a huge nostalgia rush accompanied with goosebumps.