5 - My first time defeating Master Hand in the original Smash Bros
4 - Meeting Giratina in the Shadow Dimension in Pokemon Platinum.
3 - Facing Hadron in Custom Robo Arena
2 - Bowser Lifts the Mushroom Kingdom (Opening Scene) in Paper Mario 64
1 - Greil falls to the Black Knight in Fire Emblem: Path of Radiance
edit: Honorable mention to the last stand in Halo Reach
Muda Muda Muda Muda Muda Muda!!!!