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I chose male in the poll, but only because if given a choice in a game I will generally use a male character first (if there are multiples to choose from or a method of creating the look/personality I will probably try to find/create one I identify with)

If it's something like an RPG or adventure game though and I deem it good enough to replay I will probably have playthroughs of both male and female leads.

In a game where I don't have a choice it doesn't make any difference to me while playing... I certainly wouldn't say no to a game due to a female protagonist.