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Yes, but this wouldn't be done at a national level. It won't be counted separately, all votes will go in one big pot. So, in essence, you will get some countries with an overwhelming "no", and some with an overwhelming "yes", but it doesn't matter, as the Lisbon treaty is a Europe wide thing.

You don't hold a referendum in the UK, which gets a "Yes" vote, then rejected because the "No" vote came from a very concentrated area.

You're also forgetting that not every one voted "No" in Ireland - it was a 53.4% victory. And the turnout was 53.1%. Around a quarter of the Irish electorate put a stop on a matter that effected around 700 million people.