"In Neptunes Bounty you can get an extra little sister to appear. First off, save or harvest two of the little sisters in the area. Then go get the camera and complete the Spider Splicer picture side-event. Head back to where Peachy is located, as soon as you try to enter the door you will get a message. The message tells you that you should harvest all little sisters before proceeding. DO NOT GO IN ANY FURTHER. Go back and walk around Neptunes Bounty until you see a Big Daddy with a little sister. Do not attack the Big Daddy, instead leave him. Now go look around for another Big Daddy. There should be two other Big Daddys in the area, one of them will have the extra little sister with him. You can use this one to get extra photo research and or try to harvest/save her.
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"neptunes bounty and fort frolic are both confirmed to have an "extra sister". the game glitches and gives you one too many. you'll get the message that you dont have all the sisters at the end of the level, but you can ignore it."
so yes there are 5 i believe
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