My biggest 'HOLY SHIT!' moment was actually in the original Prince of Persia for PC.
I was kinda young, and not very used to games at all, my previous experience was Super Mario Bros. and that was about it.
So anyway, I was playing all alone in the house, and I finally started to make sense of that first dungeon level. I got the sword and everything. And suddenly that skeleton lying on the ground that I've been running past all these times stands up! And it attacks me with a sword!
I kid you not, I flew up from my chair and turned the computer off, and immedeatly called my mom. Then I was lying under my covers (so to speak), afraid I would get attacked by skeletons, waiting for mom to come home. I was in a state of fear for hours, and my mom had to play the game with me for me to face my fear and get over it.
Yeah, I was like 6 or 7, but I was an impressionable child, with a vivid imagination. No game has ever replicated that fear since, but a few movies have. But that's another story.
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