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The Ghost of RubangB said:
Should I vacuum the ant poison at the same time or put some ant poison in the vacuum bag before I start the great purge?

Unless your vacuum has a hepafilter, I would not suggest putting ant poison in. You risk having some being exhausted into the air you breath.

I'd say the large quantities of dry dust in the sack will finish them off, or provide them with incentive to stay in the bag, rather than try to escape.