Half-Life 2:

"Rise and shine, Mr Freeman…rise and shine. Not that I wish to imply that you have been sleeping on the job…no one is more deserving of a rest. And all the effort in the world would have gone to waste but until…well let's just say your hour has come again. So wake up Mr Freeman, wake up and smell the ashes."

Portal... way too many to even name

"I AM TUROK!!!!"

Metal Gear Solid Series has some good ones.

I'm no hero, never was. I'm just an old killer hired to do some wet work.

-Solid Snake

I'm not going to live my life the way the boss did, I'm doing this out of loyalty to myself.

-Big Boss

War transforms us Snake, Into Beasts.

-Drebin

I can name one of Portal's quotes for you Naz.

"There was even going to be a party for you. A big party that all your friends were invited to.
I invited your best friend the companion cube. Of course, he couldn't come because you murdered him.
All your other friends couldn't come either because you don't have any other friends because of how unlikable you are. Unlikable, it says so here in your personnel file: Unlikable. Liked by no one. A bitter, unlikable loner whose passing shall not be mourned.
SHALL. NOT. BE. MOURNED. That's exactly what it says. Very formal.
Very official. It also says you're adopted, so that's funny too."

"War, war never changes..."

From Fallout 1 & 2

"my name is abobo and im gonna beat you up"
Battletoads and Double Dragon

Duke Nukem is the only correct answer.

"Blow it out your ass!" etc.

 that one is good :)

and how about this one?

 "Nobody steals our chicks, and lives!"

Leon in RE4...

"Don't worry Ashley! I'm coming for ya!"

"Saddler, you're small time."

Both of them always bring a smile to my face. :)

Battlecruiser operational - Starcraft.

I do this for Ayur - Starcraft.

Jim Raynor, here... - Starcraft.

May the rats feast on your eyes - Eternal Darkness.

The cake is a lie - Portal.