First and foremost.. I'd love to see a true return to the fundementals of classic Mario, simlar to how Prime was largely a return to form of the spirit of the original and Super Metroid games. I want fundemental, fast-paced platforming, and ditching the star fetch quest, while tightening up the controls and overall speed of the game.
In terms of the gameplay, I'd like to see some new themes, and I don't mean dry, uninspired, and repetitive concepts like galaxies, but rather, a brand new environment much like dinosaur world. At the very least, a reimagining and/or more fully fleshed out version of the mushroom kingdom would also be very interesting. I think I'd also like to see an increase in difficulty; removing over-powered powerups and/or life bars and returning to the one or two hit system would be a start.
"We hold these truths to be self-evident - all men and women created by the, go-you know.. you know the thing!" - Joe Biden